genome-based detection of novel coronavirus: an overview study

The coronavirus disease 2019, named covid-19 is a global problem. according to the declaration of the world health organization (who), it is a novel and extreme outbreak, spreading worldwide. firstly, numerous patients reported exposure in wuhan city, china at a large animals and seafood market. accordingly, the first idea is suggesting the animal-to-human transmission of this infection pathogen. next, since then, numerous patients have decelerated no exposure to animal shops, indicating that human-to-human transmission is occurring. the new coronavirus (2019-ncov) is a positive rna virus with a 29.8 kb genome and s, e, m, n, and orf1 gene fragments. the most popular method for 2019-ncov detection is genomebased approaches, like polymerase chain reaction (pcr)-based tests that require expensive experimental equipment, a controlled working environment, and high-trained technicians; they are often lacking in massive viral outbreaks. therefore, another rapid and simple genome-based assay was evaluated for this pathogen. in this review study, the rt-lamp technique, as the main isothermal amplification assays with less time consumption and without the need for expensive equipment, compared to conventional pcr-based methods to 2019-ncov identification, was discussed. accordingly, the advantage or disadvantage of these techniques was compared. the obtained data indicated that this molecular and isothermal method could be used as a successful one-step process for portable screening and the rapid identification of 2019-ncov.