haplotype effect of two human leukocyte antigen-g polymorphisms of rs1736933 and rs2735022 on the recurrent pregnancy loss

Background recurrent pregnancy loss (rpl) is a multifactorial disease that affects 1%-3% of couples.since human leukocyte antigen-g (hla-g) gene is involved in fetal maternal immune tolerance, mutationsin the hla-g gene can affect the success rate of pregnancy.objective the present study aims to investigate the haplotype effect of rs1736933 and rs2735022 polymorphismsfound in the hla-g gene on the rpl.methods in this case-control study, participants were 100 women with rpl and 80 women with normalfertility in northwestern iran. the hla-g gene promoter was amplified by polymerase chain reaction(pcr) method and sequenced. the genotype and allele frequencies of the two polymorphisms werecompared between the two groups by using t-test in spss software version 22. haplotype analysis wasperformed using phase 2.1 and haploview 4.2 applicationsfindings c allele and cc genotype in rs2735022 polymorphism and the g allele and gg genotype inrs1736933 polymorphism showed a significant association with the rpl (p<0.05). frequency of haplotypesaa, ac, ga, gc were 0.72, 0.23, 0.01, 0.03 in patients and 0.39, 0.01, 0.02, 0.59 in the control group(p<0.05). the linkage disequilibrium score was 94.conclusion analysis of gc haplotype in rs2735022 and rs1736933 polymorphisms of the hla-g gene canbe helpful in genetic studies of women with rpl.